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fitc plus anti human cd4 (Proteintech)

Name: fitc plus anti human cd4
Brand: Proteintech
Rating: 93 (26 reviews)

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Proteintech fitc plus anti human cd4

Figure 2. RNA sequencing (RNA-seq) was performed to capture differentially expressed genes in <t>CD4+</t> T cells. (A) Pearson correlation between NS and GO group samples. NS: healthy control group. (B) Reads per kilobase per million mapped reads (RPKM) distribution of NS and GO group. (C) RPKM density distribution indicated the accurate results of RNA-seq. (D) a volcano plot was presented to narrate differentially expressed genes between the NS group and the GO group (total: 679, up: 308, down: 371). (E) Gene ontology analysis was presented to demonstrate the TOP 20 signaling pathway enriched by differentially expressed genes through a dot plot. (F) Kyoto Encyclopedia of genes and Genomes (KEGG) enrichment analysis of differentially expressed genes in CD4+ T cells.

Fitc Plus Anti Human Cd4, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 26 article reviews

fitc plus anti human cd4 - by Bioz Stars, 2026-02

93/100 stars

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1) Product Images from "N6-methyladenosine modification of THBS1 induced by affluent WTAP promotes Graves' ophthalmopathy progression through glycolysis to affect Th17/Treg balance."

Article Title: N6-methyladenosine modification of THBS1 induced by affluent WTAP promotes Graves' ophthalmopathy progression through glycolysis to affect Th17/Treg balance.

Journal: Autoimmunity

doi: 10.1080/08916934.2024.2433628

Figure 2. RNA sequencing (RNA-seq) was performed to capture differentially expressed genes in CD4+ T cells. (A) Pearson correlation between NS and GO group samples. NS: healthy control group. (B) Reads per kilobase per million mapped reads (RPKM) distribution of NS and GO group. (C) RPKM density distribution indicated the accurate results of RNA-seq. (D) a volcano plot was presented to narrate differentially expressed genes between the NS group and the GO group (total: 679, up: 308, down: 371). (E) Gene ontology analysis was presented to demonstrate the TOP 20 signaling pathway enriched by differentially expressed genes through a dot plot. (F) Kyoto Encyclopedia of genes and Genomes (KEGG) enrichment analysis of differentially expressed genes in CD4+ T cells.

Figure Legend Snippet: Figure 2. RNA sequencing (RNA-seq) was performed to capture differentially expressed genes in CD4+ T cells. (A) Pearson correlation between NS and GO group samples. NS: healthy control group. (B) Reads per kilobase per million mapped reads (RPKM) distribution of NS and GO group. (C) RPKM density distribution indicated the accurate results of RNA-seq. (D) a volcano plot was presented to narrate differentially expressed genes between the NS group and the GO group (total: 679, up: 308, down: 371). (E) Gene ontology analysis was presented to demonstrate the TOP 20 signaling pathway enriched by differentially expressed genes through a dot plot. (F) Kyoto Encyclopedia of genes and Genomes (KEGG) enrichment analysis of differentially expressed genes in CD4+ T cells.

Techniques Used: RNA Sequencing, Control

Figure 3. Analysis of methylation differences in CD4+ T cells between NS group and GO group. (A) The distribution of m6A peak density from GO along a meta gene. (B) Presentation of differences in CDS, stop C, 5’UTR, 3’UTR, and start C occupancy between the two groups. (C) Motif sequences for GO group and NS group. (D) A total of 3277 m6A sites (hypermethylated _ sites 1302, hypomethylated _ sites, 1975) were identified by MeRIP-seq. (E) 1302 hypermethylated _ sites corresponded to 1143 genes and 1975 hypomethylated _ sites corresponded to 1601 genes. (F) The chromosomal distribution of corresponding m6A peaks. Hype _ sites: hypermethylated _ sites; hypo _ sites: Hypomethylated _ sites.

Figure Legend Snippet: Figure 3. Analysis of methylation differences in CD4+ T cells between NS group and GO group. (A) The distribution of m6A peak density from GO along a meta gene. (B) Presentation of differences in CDS, stop C, 5’UTR, 3’UTR, and start C occupancy between the two groups. (C) Motif sequences for GO group and NS group. (D) A total of 3277 m6A sites (hypermethylated _ sites 1302, hypomethylated _ sites, 1975) were identified by MeRIP-seq. (E) 1302 hypermethylated _ sites corresponded to 1143 genes and 1975 hypomethylated _ sites corresponded to 1601 genes. (F) The chromosomal distribution of corresponding m6A peaks. Hype _ sites: hypermethylated _ sites; hypo _ sites: Hypomethylated _ sites.

Techniques Used: Methylation

Figure 4. Functional analysis of hypermethylated and hypomethylated genes between NS group and GO group. (A) Gene ontology enrichment analysis of hyper methylated genes in CD4+ T cells of GO group. (B) KEGG enrichment analysis of hypermethylated genes in GO group. (C) Gene ontology enrichment analysis of hypomethylated genes in GO group. (D) KEGG enrichment analysis of hypomethylated genes in GO group.

Figure Legend Snippet: Figure 4. Functional analysis of hypermethylated and hypomethylated genes between NS group and GO group. (A) Gene ontology enrichment analysis of hyper methylated genes in CD4+ T cells of GO group. (B) KEGG enrichment analysis of hypermethylated genes in GO group. (C) Gene ontology enrichment analysis of hypomethylated genes in GO group. (D) KEGG enrichment analysis of hypomethylated genes in GO group.

Techniques Used: Functional Assay, Methylation

Figure 5. MeRIP-seq combined with RNA-seq reveals THBS1 as a target gene in CD4+ T cells of GO. (A) Veen diagram presented the number of intersecting genes according to MeRIP-seq and RNA-seq. Hypermethylated and upregulated genes: 81; hypermethylated and downregulated genes: 4; hypomethylated and upregu lated genes: 6; hypomethylated and downregulated genes: 121. (B) TOP 6 mRNA genes (FGF11, PRKCZ, GNG4, PDGFB, THBS1, and FN1) which were characterized with hypermethylation and up-regulated were lying in the PI3K-Akt signaling pathway. (C) The expression maps of 6 candidate genes (FGF11, PRKCZ, GNG4, PDGFB, THBS1, and FN1) were detected by RT-qPCR. (D) The knockdown efficiency of si-THBS1-1 (P = 0.008), si-THBS1-2 (P = 0.006), and si-THBS1-3 (P = 0.001) was presented by RT-qPCR results. The knock-down efficiency of si-THBS1-3 (p = 0.001) is the most desirable. (E) Western blot verified the knockdown effect of si-THBS1-3. (F) The lactic acid content in CD4+T cells was significantly reduced after the silence of THBS1. (G) Glucose uptake in CD4+T cells was subsequently reduced by THBS1 knockdown. *P < 0.05; **P < 0.01; ***P < 0.001.

Figure Legend Snippet: Figure 5. MeRIP-seq combined with RNA-seq reveals THBS1 as a target gene in CD4+ T cells of GO. (A) Veen diagram presented the number of intersecting genes according to MeRIP-seq and RNA-seq. Hypermethylated and upregulated genes: 81; hypermethylated and downregulated genes: 4; hypomethylated and upregu lated genes: 6; hypomethylated and downregulated genes: 121. (B) TOP 6 mRNA genes (FGF11, PRKCZ, GNG4, PDGFB, THBS1, and FN1) which were characterized with hypermethylation and up-regulated were lying in the PI3K-Akt signaling pathway. (C) The expression maps of 6 candidate genes (FGF11, PRKCZ, GNG4, PDGFB, THBS1, and FN1) were detected by RT-qPCR. (D) The knockdown efficiency of si-THBS1-1 (P = 0.008), si-THBS1-2 (P = 0.006), and si-THBS1-3 (P = 0.001) was presented by RT-qPCR results. The knock-down efficiency of si-THBS1-3 (p = 0.001) is the most desirable. (E) Western blot verified the knockdown effect of si-THBS1-3. (F) The lactic acid content in CD4+T cells was significantly reduced after the silence of THBS1. (G) Glucose uptake in CD4+T cells was subsequently reduced by THBS1 knockdown. *P < 0.05; **P < 0.01; ***P < 0.001.

Techniques Used: RNA Sequencing, Expressing, Quantitative RT-PCR, Knockdown, Western Blot

Figure 6. WTAP is abnormal in CD4+ T cells of GO and regulates the m6A level and expression of THBS1. (A) Potential m6A loci on THBS1 sequence between NS group and GO group. (B) The expression of four common m6A modifying enzymes (METTL3, METTL14, WTAP, KIAA1429) was verified by RT-qPCR, only WTAP was significantly highly expressed. (C) The interfered effect of si-WTAP-1 (P = 0.001), si-WTAP-2 (P = 0.001), and si-WTAP-3 (P = 0) on WTAP was confirmed by RT-qPCR, and si-WTAP-3 was selected for the subsequent experiment. (D) The sequence motif (GGACA) of WTAP was in accord with “RRACH”. (E) Silencing of WTAP signifi cantly reduced the expression level of THBS1 compared with the control group. (F) MeRIP-PCR showed that the knockdown of WTAP decreased the m6A abun dance of THBS1. *P < 0.05; **P < 0.01; ***P < 0.001.

Figure Legend Snippet: Figure 6. WTAP is abnormal in CD4+ T cells of GO and regulates the m6A level and expression of THBS1. (A) Potential m6A loci on THBS1 sequence between NS group and GO group. (B) The expression of four common m6A modifying enzymes (METTL3, METTL14, WTAP, KIAA1429) was verified by RT-qPCR, only WTAP was significantly highly expressed. (C) The interfered effect of si-WTAP-1 (P = 0.001), si-WTAP-2 (P = 0.001), and si-WTAP-3 (P = 0) on WTAP was confirmed by RT-qPCR, and si-WTAP-3 was selected for the subsequent experiment. (D) The sequence motif (GGACA) of WTAP was in accord with “RRACH”. (E) Silencing of WTAP signifi cantly reduced the expression level of THBS1 compared with the control group. (F) MeRIP-PCR showed that the knockdown of WTAP decreased the m6A abun dance of THBS1. *P < 0.05; **P < 0.01; ***P < 0.001.

Techniques Used: Expressing, Sequencing, Quantitative RT-PCR, Control, Knockdown

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Journal: Autoimmunity

Article Title: N6-methyladenosine modification of THBS1 induced by affluent WTAP promotes Graves' ophthalmopathy progression through glycolysis to affect Th17/Treg balance.

doi: 10.1080/08916934.2024.2433628

Figure Lengend Snippet: Figure 2. RNA sequencing (RNA-seq) was performed to capture differentially expressed genes in CD4+ T cells. (A) Pearson correlation between NS and GO group samples. NS: healthy control group. (B) Reads per kilobase per million mapped reads (RPKM) distribution of NS and GO group. (C) RPKM density distribution indicated the accurate results of RNA-seq. (D) a volcano plot was presented to narrate differentially expressed genes between the NS group and the GO group (total: 679, up: 308, down: 371). (E) Gene ontology analysis was presented to demonstrate the TOP 20 signaling pathway enriched by differentially expressed genes through a dot plot. (F) Kyoto Encyclopedia of genes and Genomes (KEGG) enrichment analysis of differentially expressed genes in CD4+ T cells.

Article Snippet: For the Treg cells, FITC plus anti-human CD4 (Proteintech, FITC-65134), PE anti-human CD25 antibody (Biolegend, 302605), Alexa Fluor® 647 anti-mouse/rat/human FOXP3 antibody (Biolegend, 320013) were applied.

Techniques: RNA Sequencing, Control

Journal: Autoimmunity

Article Title: N6-methyladenosine modification of THBS1 induced by affluent WTAP promotes Graves' ophthalmopathy progression through glycolysis to affect Th17/Treg balance.

doi: 10.1080/08916934.2024.2433628

Figure Lengend Snippet: Figure 3. Analysis of methylation differences in CD4+ T cells between NS group and GO group. (A) The distribution of m6A peak density from GO along a meta gene. (B) Presentation of differences in CDS, stop C, 5’UTR, 3’UTR, and start C occupancy between the two groups. (C) Motif sequences for GO group and NS group. (D) A total of 3277 m6A sites (hypermethylated _ sites 1302, hypomethylated _ sites, 1975) were identified by MeRIP-seq. (E) 1302 hypermethylated _ sites corresponded to 1143 genes and 1975 hypomethylated _ sites corresponded to 1601 genes. (F) The chromosomal distribution of corresponding m6A peaks. Hype _ sites: hypermethylated _ sites; hypo _ sites: Hypomethylated _ sites.

Techniques: Methylation

Journal: Autoimmunity

Article Title: N6-methyladenosine modification of THBS1 induced by affluent WTAP promotes Graves' ophthalmopathy progression through glycolysis to affect Th17/Treg balance.

doi: 10.1080/08916934.2024.2433628

Figure Lengend Snippet: Figure 4. Functional analysis of hypermethylated and hypomethylated genes between NS group and GO group. (A) Gene ontology enrichment analysis of hyper methylated genes in CD4+ T cells of GO group. (B) KEGG enrichment analysis of hypermethylated genes in GO group. (C) Gene ontology enrichment analysis of hypomethylated genes in GO group. (D) KEGG enrichment analysis of hypomethylated genes in GO group.

Techniques: Functional Assay, Methylation

Journal: Autoimmunity

Article Title: N6-methyladenosine modification of THBS1 induced by affluent WTAP promotes Graves' ophthalmopathy progression through glycolysis to affect Th17/Treg balance.

doi: 10.1080/08916934.2024.2433628

Figure Lengend Snippet: Figure 5. MeRIP-seq combined with RNA-seq reveals THBS1 as a target gene in CD4+ T cells of GO. (A) Veen diagram presented the number of intersecting genes according to MeRIP-seq and RNA-seq. Hypermethylated and upregulated genes: 81; hypermethylated and downregulated genes: 4; hypomethylated and upregu lated genes: 6; hypomethylated and downregulated genes: 121. (B) TOP 6 mRNA genes (FGF11, PRKCZ, GNG4, PDGFB, THBS1, and FN1) which were characterized with hypermethylation and up-regulated were lying in the PI3K-Akt signaling pathway. (C) The expression maps of 6 candidate genes (FGF11, PRKCZ, GNG4, PDGFB, THBS1, and FN1) were detected by RT-qPCR. (D) The knockdown efficiency of si-THBS1-1 (P = 0.008), si-THBS1-2 (P = 0.006), and si-THBS1-3 (P = 0.001) was presented by RT-qPCR results. The knock-down efficiency of si-THBS1-3 (p = 0.001) is the most desirable. (E) Western blot verified the knockdown effect of si-THBS1-3. (F) The lactic acid content in CD4+T cells was significantly reduced after the silence of THBS1. (G) Glucose uptake in CD4+T cells was subsequently reduced by THBS1 knockdown. *P < 0.05; **P < 0.01; ***P < 0.001.

Techniques: RNA Sequencing, Expressing, Quantitative RT-PCR, Knockdown, Western Blot

Journal: Autoimmunity

Article Title: N6-methyladenosine modification of THBS1 induced by affluent WTAP promotes Graves' ophthalmopathy progression through glycolysis to affect Th17/Treg balance.

doi: 10.1080/08916934.2024.2433628

Figure Lengend Snippet: Figure 6. WTAP is abnormal in CD4+ T cells of GO and regulates the m6A level and expression of THBS1. (A) Potential m6A loci on THBS1 sequence between NS group and GO group. (B) The expression of four common m6A modifying enzymes (METTL3, METTL14, WTAP, KIAA1429) was verified by RT-qPCR, only WTAP was significantly highly expressed. (C) The interfered effect of si-WTAP-1 (P = 0.001), si-WTAP-2 (P = 0.001), and si-WTAP-3 (P = 0) on WTAP was confirmed by RT-qPCR, and si-WTAP-3 was selected for the subsequent experiment. (D) The sequence motif (GGACA) of WTAP was in accord with “RRACH”. (E) Silencing of WTAP signifi cantly reduced the expression level of THBS1 compared with the control group. (F) MeRIP-PCR showed that the knockdown of WTAP decreased the m6A abun dance of THBS1. *P < 0.05; **P < 0.01; ***P < 0.001.

Techniques: Expressing, Sequencing, Quantitative RT-PCR, Control, Knockdown

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